|
| Status |
Public on May 04, 2023 |
| Title |
mESC_Mdivi1_24h rep3 |
| Sample type |
SRA |
| |
|
| Source name |
embryonic stem cells (ESCs)
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129S5/SvEvBrd
cell type: ESCs
genotype: Mdivi1 treatment
|
| Treatment protocol |
ESCs were treated with 50 μM Mdivi-1 for 24h.
|
| Growth protocol |
The mouse ESC line AB2.2 were seeded on mitotically inactivated MEF feeder cells in N2B27 medium with 2i and LIF in tissue culture dishes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For mouse ESCs, total RNA was extracted by TRIzol reagent (Invitrogen, USA) from 5×105 cells for each group.
RNA libraries were prepared for sequencing using standard RNA-seq library preparation (Truseq RNA Sample Preparation Kit, Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Raw reads were processed with Cutadapt v1.16. Trimmed reads were mapped to mouse genome using STAR with default settings.
Assembly: mm10
Supplementary files format and content: DESeq result with normalized and raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Dec 29, 2022 |
| Last update date |
May 04, 2023 |
| Contact name |
Li-quan Zhou |
| E-mail(s) |
zhouliquan@hust.edu.cn
|
| Phone |
+86-27-83692651
|
| Organization name |
Huazhong University of Science and Technology
|
| Department |
Tongji Medical College
|
| Lab |
ZhouLab
|
| Street address |
13 Hangkong road
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430030 |
| Country |
China |
| |
|
| Platform ID |
GPL21493 |
| Series (2) |
| GSE221885 |
Mdivi1 treatment impacts transcriptome of mouse embryonic stem cells |
| GSE221989 |
Mdivi1 treatment impacts mouse embryonic stem cells and early embryos. |
|
| Relations |
| BioSample |
SAMN32489897 |
| SRA |
SRX18881982 |
