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| Status |
Public on Sep 06, 2023 |
| Title |
CAE patient [CAE5] |
| Sample type |
SRA |
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| Source name |
venous blood
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| Organism |
Homo sapiens |
| Characteristics |
cell type: venous blood cells
Sex: Female
age: 72
tissue: venous blood
bmi: 25.8
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from peripheral venous blood samples by using the Trizol reagent.
RNA libraries for RNA-seq were prepared using RNeasy Mini Kit. following manufacturer's protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
The quality and quantity of RNA were determined on a Nanodrop ND-1000 spectrophotometer. Agilent 2100 Bioanalyzer was used to determine the quality of the sequencing library.
The Illumina HiSeq platform was applied to perform RNA sequencing. The original image data obtained by Illumina sequencing was converted into sequence data (FASTQ format) through Base Calling.
Trimmomatic software was used to preprocess the original data, including removal of adaptor, low quality reads and the low quality bases from the 3' end and 5' end in different ways.
The original sequencing volume, effective sequencing volume, Q30 and GC content were counted and comprehensively evaluated. After quality control, the high-quality sequence was compared with the specified reference genome (GRCh38) by hisat2.
Assembly: hg18
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
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| Submission date |
Jan 02, 2023 |
| Last update date |
Sep 06, 2023 |
| Contact name |
Xiao Bing |
| E-mail(s) |
xiaobing@hb2h.com
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| Organization name |
The Second Hospital of Hebei Medical University
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| Street address |
No.215, Heping West Road, Shijiazhuang, Hebei, China
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| City |
Shijiazhuang |
| ZIP/Postal code |
050000 |
| Country |
China |
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| Platform ID |
GPL16791 |
| Series (1) |
| GSE222041 |
Identification of potential abnormal methylation modified genes in coronary artery ectasia |
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| Relations |
| BioSample |
SAMN32537742 |
| SRA |
SRX18910064 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6912194_CAE5_processed.txt.gz |
203.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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