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Sample GSM692509 Query DataSets for GSM692509
Status Public on Aug 25, 2011
Title Ep300 hetHSC sample 2 replicate 1
Sample type RNA
 
Source name Sca-1+,Lin-,AA4.1+,c-Kit++ FLHSC from 15 embryos combined
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: Ep300+/- FLHSC
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy micro kit following manufacturer's protocol.
12ng of total RNA was amplified following the Ovation RNA amplification protocol (FLHSC).
Label Biotin
Label protocol 12 nanograms of total RNA was amplified and processed to produce biotinylated cDNA targets using the NuGen Ovation Biotin labeling system.
 
Hybridization protocol 3.75 micrograms of biotinylated material was hybridized according to the standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol
Description 14.5dpc fetal liver HSC
Data processing GCRMA probe summarization and normalization using the Bioconductor gcrma package (http://www.bioconductor.org).
 
Submission date Mar 16, 2011
Last update date Aug 25, 2011
Contact name Madeleine E. Lemieux
E-mail(s) mlemieux@bioinfo.ca
Phone 617-595-6695
URL http://www.bioinfo.ca
Organization name Bioinfo
Street address 275 Main St., Suite 252
City Plantagenet
State/province ON
ZIP/Postal code K0B 1L0
Country Canada
 
Platform ID GPL1261
Series (1)
GSE27987 Differential pre-mRNA processing in Crebbp+/- mice

Data table header descriptions
ID_REF
VALUE log2 GCRMA-normalized intensity

Data table
ID_REF VALUE
1415670_at 8.098
1415671_at 8.347
1415672_at 10.416
1415673_at 7.839
1415674_a_at 8.103
1415675_at 10.066
1415676_a_at 11.75
1415677_at 7.242
1415678_at 11.914
1415679_at 11.049
1415680_at 9.18
1415681_at 9.405
1415682_at 8.532
1415683_at 8.946
1415684_at 7.422
1415685_at 8.814
1415686_at 11.198
1415687_a_at 10.257
1415688_at 10.73
1415689_s_at 6.543

Total number of rows: 45101

Table truncated, full table size 765 Kbytes.




Supplementary file Size Download File type/resource
GSM692509_P300_hetHSC_2_1.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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