|
| Status |
Public on Aug 25, 2011 |
| Title |
Cdkn1a nullHSC 1 |
| Sample type |
RNA |
| |
|
| Source name |
Sca-1+,Lin-,AA4.1+,c-Kit++ FLHSC from 15 embryos combined
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6/129Sv
cell type: Cdkn1a-/- FLHSC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy micro kit following manufacturer's protocol.
12ng of total RNA was amplified following the Ovation RNA amplification protocol (FLHSC).
|
| Label |
Biotin
|
| Label protocol |
12 nanograms of total RNA was amplified and processed to produce biotinylated cDNA targets using the NuGen Ovation Biotin labeling system.
|
| |
|
| Hybridization protocol |
3.75 micrograms of biotinylated material was hybridized according to the standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
14.5dpc fetal liver HSC
|
| Data processing |
GCRMA probe summarization and normalization using the Bioconductor gcrma package (http://www.bioconductor.org).
|
| |
|
| Submission date |
Mar 16, 2011 |
| Last update date |
Aug 25, 2011 |
| Contact name |
Madeleine E. Lemieux |
| E-mail(s) |
mlemieux@bioinfo.ca
|
| Phone |
617-595-6695
|
| URL |
http://www.bioinfo.ca
|
| Organization name |
Bioinfo
|
| Street address |
275 Main St., Suite 252
|
| City |
Plantagenet |
| State/province |
ON |
| ZIP/Postal code |
K0B 1L0 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27987 |
Differential pre-mRNA processing in Crebbp+/- mice |
|
