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Sample GSM6928922 Query DataSets for GSM6928922
Status Public on Nov 30, 2023
Title HH 2
Sample type RNA
 
Source name HH 2
Organism Homo sapiens
Characteristics treatment: HH
Treatment protocol 2h synchronization (100nM dexamethasone) then 1µM homoharringtonine or 100nM Rocaglamide A during 4h. Vehicule = 0,1% DMSO
Growth protocol DMEM/F12 [D6434] + L-glutamine + P/S + 12,5% SVF
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Nucleospin RNA kit from Macherey Nagel according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 300ng total RNA (GeneChip® WT PLUS Reagent Kit, Affymetrix).
 
Hybridization protocol Following fragmentation, 2,3 microgrammes of ssDNA were hybridized for 16 hr at 45C on Clariom S Human Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip Scanner GCS3000.
Data processing data were processed using our in-house Galaxy instance with “GIANT” (Galaxy-based interactive tools for Analysis of Transcriptomic data). It consists of modules allowing to perform quality control (QC), Robust Multi-Average method normalization, LIMMA differential analysis using apt-probeset-summarize tool (v.2.10.2). Normalization done on gene level with NetAffx Annotation Release 36.
Clariom_S_Human.r1.pgf
Clariom_S_Human.r1.na36.hg38.transcript.csv
Log2 RMA
 
Submission date Jan 11, 2023
Last update date Nov 30, 2023
Contact name Alexandre Berthier
E-mail(s) alexandre.berthier@inserm.fr
Phone 0320974217
Organization name Inserm
Street address U1011, Bâtiment J&K, Bd du Pr Leclerc
City Lille
ZIP/Postal code 59000
Country France
 
Platform ID GPL23159
Series (1)
GSE222682 Effect of homoharringtonine and Rocaglamide A on synchronized AC16 cells

Supplementary file Size Download File type/resource
GSM6928922_5_HH-2_Clariom_S_Human_.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data are available on Series record

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