|
Status |
Public on Jan 12, 2023 |
Title |
Prototype Sample APS-1 (1) |
Sample type |
protein |
|
|
Source name |
Serum/Plasma
|
Organism |
Homo sapiens |
Characteristics |
disease: Prototype Autoimmune Disease Sample
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
protein |
Extraction protocol |
Blood was collected from patients and healthy controls. The blood was then processed into serum or plasma which was then run on our arrays.
|
Label |
R-PE conjugated goat anti-human IgG secondary antibody
|
Label protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
|
|
Hybridization protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
Scan protocol |
A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
|
Description |
Antibodies in samples bind to conjugated beads in array. The fluorescence measured as MFI from the secondary antibody was used to determine antibody reactivity.
|
Data processing |
Sample replicate MFI values were averaged for each antigen. The average barebead MFI was then subtracted from the average MFI of each sample and antigen.
|
|
|
Submission date |
Jan 12, 2023 |
Last update date |
Jan 12, 2023 |
Contact name |
Allan Feng |
E-mail(s) |
afeng1@stanford.edu
|
Phone |
2055678956
|
Organization name |
Stanford School of Medicine
|
Department |
Imm/Rheum
|
Lab |
Utz
|
Street address |
269 Campus Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL33017 |
Series (2) |
GSE222760 |
Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness [ACA] |
GSE222765 |
Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness |
|