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Sample GSM6931467 Query DataSets for GSM6931467
Status Public on Jan 12, 2023
Title Prototype Sample APS-1 (1)
Sample type protein
 
Source name Serum/Plasma
Organism Homo sapiens
Characteristics disease: Prototype Autoimmune Disease Sample
Treatment protocol NA
Growth protocol NA
Extracted molecule protein
Extraction protocol Blood was collected from patients and healthy controls. The blood was then processed into serum or plasma which was then run on our arrays.
Label R-PE conjugated goat anti-human IgG secondary antibody
Label protocol A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
 
Hybridization protocol A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
Scan protocol A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
Description Antibodies in samples bind to conjugated beads in array. The fluorescence measured as MFI from the secondary antibody was used to determine antibody reactivity.
Data processing Sample replicate MFI values were averaged for each antigen. The average barebead MFI was then subtracted from the average MFI of each sample and antigen.
 
Submission date Jan 12, 2023
Last update date Jan 12, 2023
Contact name Allan Feng
E-mail(s) afeng1@stanford.edu
Phone 2055678956
Organization name Stanford School of Medicine
Department Imm/Rheum
Lab Utz
Street address 269 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL33017
Series (2)
GSE222760 Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness [ACA]
GSE222765 Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness

Data table header descriptions
ID_REF
VALUE Each numerical value represents the median fluorescent intensity (MFI) of each sample and bead after data processing.

Data table
ID_REF VALUE
6 0
7 0
8 1300
9 5333.25
10 9209.5
11 4422.5
12 6985.25
13 4241.25
14 3679.5
15 0
16 0
17 0
18 0
19 0
20 0
21 0
22 0
23 0
24 0
25 0

Total number of rows: 58

Table truncated, full table size <1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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