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Sample GSM6946147 Query DataSets for GSM6946147
Status Public on May 29, 2024
Title RBG26221_Plate_1_KO
Sample type SRA
 
Source name bone marrow
Organism Mus musculus
Characteristics tissue: bone marrow
cell type: ESLAM
genotype: KO
Extracted molecule total RNA
Extraction protocol Bone marrow cells were harvested from STAT5KO and WT control mice, single ESLAM HSCs was FACS sorted into 2.3ul 0.2% Triton-100 containg RNAse inhibitor
Tagmentation is carried out using the Illumina Nextera XT DNA sample preparation kit. The protocol is based on the Tagmentation protocol from Fluidigm.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description SLX.14260.i710_i502
Data processing RNA-seq reads were aligned to mm10 + ERCC92 using GSNAP (version 2018-03-25) with parameters (-B 5 -t 24 -n 1 -Q -N 1).
Reads in features were counted with htseq-count (HTSeq version 0.9.1) with the parameter (-s no).
Assembly: GRCm38.81
Supplementary files format and content: HTSeq counts text file with raw counts and htseq metrics
Supplementary files format and content: Anndata h5 file with filtered cells and genes and data transformations (in layers)
 
Submission date Jan 20, 2023
Last update date May 29, 2024
Contact name Hugo P. Bastos
E-mail(s) hpb29@cam.ac.uk
Organization name Wellcome - MRC Cambridge Stem Cell Institute
Department Haematology
Street address Jeffrey Cheah Biomedical Centre, Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
 
Platform ID GPL17021
Series (1)
GSE223366 Maintenance of ESLAM haematopoietic stem cells by tyrosine-unphosphorylated STAT5 and JAK inhibition
Relations
BioSample SAMN32815208
SRA SRX19107416

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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