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Sample GSM6946155 Query DataSets for GSM6946155
Status Public on May 29, 2024
Title RBG26352_Plate_3_KO
Sample type SRA
Source name bone marrow
Organism Mus musculus
Characteristics tissue: bone marrow
cell type: ESLAM
genotype: KO
Extracted molecule total RNA
Extraction protocol Bone marrow cells were harvested from STAT5KO and WT control mice, single ESLAM HSCs was FACS sorted into 2.3ul 0.2% Triton-100 containg RNAse inhibitor
Tagmentation is carried out using the Illumina Nextera XT DNA sample preparation kit. The protocol is based on the Tagmentation protocol from Fluidigm.
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description SLX.14260.i707_i517
Data processing RNA-seq reads were aligned to mm10 + ERCC92 using GSNAP (version 2018-03-25) with parameters (-B 5 -t 24 -n 1 -Q -N 1).
Reads in features were counted with htseq-count (HTSeq version 0.9.1) with the parameter (-s no).
Assembly: GRCm38.81
Supplementary files format and content: HTSeq counts text file with raw counts and htseq metrics
Supplementary files format and content: Anndata h5 file with filtered cells and genes and data transformations (in layers)
Submission date Jan 20, 2023
Last update date May 29, 2024
Contact name Hugo P. Bastos
Organization name Wellcome - MRC Cambridge Stem Cell Institute
Department Haematology
Street address Jeffrey Cheah Biomedical Centre, Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
Platform ID GPL17021
Series (1)
GSE223366 Maintenance of ESLAM haematopoietic stem cells by tyrosine-unphosphorylated STAT5 and JAK inhibition
BioSample SAMN32815200
SRA SRX19107424

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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