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Sample GSM6947318 Query DataSets for GSM6947318
Status Public on Oct 01, 2023
Title Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV _rep3
Sample type RNA
Source name extracellular vesicles (EVs)
Organism Homo sapiens
Characteristics cell type: Bone Marrow Stem Cells (BMSCs)
tissue: extracellular vesicles (EVs)
treatment: Plasmids cd64_TP53 treated
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
Label Cy3
Label protocol For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
Hybridization protocol The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays (SurePrint G3 Human CGH Microarray 8x60K V2 ) at 45 °C for 16 hrs.
Scan protocol Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
Description Plasmids cd64_TP53 treated BMSC-derived EV
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Jan 20, 2023
Last update date Oct 02, 2023
Contact name Inyoul Lee
Organization name Institute for Systems Biology
Street address 401 Terry Ave. North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL16699
Series (2)
GSE223407 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Human Array]
GSE223409 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer

Data table header descriptions
VALUE Quantile normalized gMean signal intensity (log2)

Data table
1 11.97227304
2 5.241631491
3 5.303751626
4 10.50117451
5 6.770372103
6 5.341386515
7 5.552622111
8 5.532525451
9 10.70544003
10 8.01117304
11 5.570111788
12 7.400716411
13 6.174526145
14 5.390559378
15 5.215436911
16 5.504021444
17 6.843488603
18 7.025521336
19 7.834522078
20 10.1005314

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM6947318_253949451908_1_1_BMSC_64_53_EV_rep3.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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