|Public on Oct 01, 2023
|Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV _rep3
|extracellular vesicles (EVs)
|cell type: Bone Marrow Stem Cells (BMSCs)
tissue: extracellular vesicles (EVs)
treatment: Plasmids cd64_TP53 treated
|Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
|For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
|The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays (SurePrint G3 Human CGH Microarray 8x60K V2 ) at 45 °C for 16 hrs.
|Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
|Plasmids cd64_TP53 treated BMSC-derived EV
|The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|Jan 20, 2023
|Last update date
|Oct 02, 2023
|Institute for Systems Biology
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|Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Human Array]
|Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer