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Sample GSM6947334 Query DataSets for GSM6947334
Status Public on Oct 01, 2023
Title Gene expression of Plasmids cd64_TP53 treated MEF-derived EV_rep1
Sample type RNA
 
Source name extracellular vesicles (EVs)
Organism Mus musculus
Characteristics cell type: Mouse Embryonic Fibroblast (MEF)
tissue: extracellular vesicles (EVs)
treatment: Plasmids cd64_TP53 treated
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
Label Cy3
Label protocol For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
 
Hybridization protocol The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays at 45 °C for 16 hrs.
Scan protocol Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
Description Plasmids cd64_TP53 treated MEF-derived EV
MEF_64_53_rep1
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jan 20, 2023
Last update date Oct 02, 2023
Contact name Inyoul Lee
Organization name Institute for Systems Biology
Street address 401 Terry Ave. North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL13912
Series (2)
GSE223408 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Mouse Array]
GSE223409 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer

Data table header descriptions
ID_REF
VALUE Quantile normalized gMean signal intensity (log2)

Data table
ID_REF VALUE
1 13.21636591
2 5.642077415
3 5.733667833
4 5.575444703
5 5.829727681
6 5.959641486
7 5.613249544
8 5.649403176
9 5.567037726
10 5.799702364
11 5.730212829
12 5.799295186
13 7.268688653
14 5.9053248
15 5.938684105
16 5.720922499
17 5.711849234
18 5.936032705
19 5.857083171
20 6.076862846

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM6947334_252800513092_1_2_MEF_64_53_EV_rep1.txt.gz 11.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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