NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM6947335 Query DataSets for GSM6947335
Status Public on Oct 01, 2023
Title Gene expression of PBS treated MEF-derived EV_rep2
Sample type RNA
 
Source name extracellular vesicles (EVs)
Organism Mus musculus
Characteristics cell type: Mouse Embryonic Fibroblast (MEF)
tissue: extracellular vesicles (EVs)
treatment: PBS treated
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
Label Cy3
Label protocol For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
 
Hybridization protocol The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays at 45 °C for 16 hrs.
Scan protocol Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
Description PBS treated MEF-derived EV
MEF_PBS_EV_rep2
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jan 20, 2023
Last update date Oct 02, 2023
Contact name Inyoul Lee
Organization name Institute for Systems Biology
Street address 401 Terry Ave. North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL13912
Series (2)
GSE223408 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Mouse Array]
GSE223409 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer

Data table header descriptions
ID_REF
VALUE Quantile normalized gMean signal intensity (log2)

Data table
ID_REF VALUE
1 9.853442281
2 5.806359524
3 5.8337782
4 5.633438974
5 6.055021053
6 5.956944137
7 5.768798795
8 6.354252443
9 5.661583751
10 5.799288783
11 5.809462569
12 5.677484111
13 6.367592064
14 9.347702866
15 6.034925281
16 5.645679819
17 8.467637056
18 6.365548804
19 7.875235848
20 11.16758488

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM6947335_252800513092_1_3_MEF_PBS_EV_rep2.txt.gz 12.0 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap