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Sample GSM6947340 Query DataSets for GSM6947340
Status Public on Oct 01, 2023
Title Gene expression of Control MEF-derived EV_rep1
Sample type RNA
 
Source name extracellular vesicles (EVs)
Organism Mus musculus
Characteristics cell type: Mouse Embryonic Fibroblast (MEF)
tissue: extracellular vesicles (EVs)
treatment: Control
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
Label Cy3
Label protocol For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
 
Hybridization protocol The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays at 45 °C for 16 hrs.
Scan protocol Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
Description Control MEF-derived EV
MEF_Ctrl_EV_rep1
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jan 20, 2023
Last update date Oct 02, 2023
Contact name Inyoul Lee
Organization name Institute for Systems Biology
Street address 401 Terry Ave. North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL13912
Series (2)
GSE223408 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Mouse Array]
GSE223409 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer

Data table header descriptions
ID_REF
VALUE Quantile normalized gMean signal intensity (log2)

Data table
ID_REF VALUE
1 9.819823642
2 5.472159461
3 5.859823412
4 5.715684903
5 5.880864976
6 5.756024055
7 5.791627156
8 7.264082864
9 5.689742525
10 5.751020768
11 5.846151693
12 5.902610268
13 6.688029953
14 9.154571011
15 5.862444404
16 5.633611245
17 7.683950046
18 6.072750146
19 7.296605779
20 10.10956496

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM6947340_252800513092_2_4_MEF_Ctrl_EV_rep1.txt.gz 11.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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