|
| Status |
Public on Oct 01, 2023 |
| Title |
Gene expression of PBS treated MEF-derived EV_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
extracellular vesicles (EVs)
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mouse Embryonic Fibroblast (MEF)
tissue: extracellular vesicles (EVs)
treatment: PBS treated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
|
| |
|
| Hybridization protocol |
The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays at 45 °C for 16 hrs.
|
| Scan protocol |
Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
|
| Description |
PBS treated MEF-derived EV
MEF_PBS_EV_rep5
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jan 20, 2023 |
| Last update date |
Oct 02, 2023 |
| Contact name |
Inyoul Lee |
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Ave. North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL13912 |
| Series (2) |
| GSE223408 |
Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Mouse Array] |
| GSE223409 |
Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer |
|
