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Sample GSM6951244 Query DataSets for GSM6951244
Status Public on Jun 07, 2023
Title Dcir-/- osteoclast 83
Sample type SRA
Source name Osteoclasts
Organism Mus musculus
Characteristics strain: C57BL/6J
cell type: Osteoclasts
time: day 4
genotype: Dcir-/-
treatment: RANKL stimulation for 2 days
Treatment protocol Osteoclasts were induced in the presence of M-CSF (20 ng/ml) and RANKL (100 ng/ml) for 2 days cutlure.
Growth protocol Bone marrow cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
Extracted molecule total RNA
Extraction protocol Total RNA was harvested using FastGeneTM RNA basic kit (NIPPON Genetics). The quality of RNA was determned by the TapeStation system. 1 mg of total RNA was used for the construction of sequencing libraries.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description OC83
Data processing The raw sequenceing data were processed using TrimGalore v0.6.6 to remove adapter sequences and low-quality reads.
The processed reads were alined to the mouse genome reference mm10 using hisat2 v2.2.0.
Aligned read counts were caluculated by R package Rsubread v1.32.4.
Assembly: mm10
Supplementary files format and content: An Excel file include differential gene values and p-value.
Submission date Jan 21, 2023
Last update date Jun 07, 2023
Contact name Tomonori Kaifu
Phone +817270212
Organization name Tohoku Medicine and Pharmaceutical University
Department Faculty of Medicine
Lab Division of Immunology
Street address 1-15-1 Fukumuro Miyagino ku
City Sendai
State/province Miyagi
ZIP/Postal code 9838536
Country Japan
Platform ID GPL24247
Series (1)
GSE223429 Gene expression of Dcir-/- osteoclasts induced by M-CSF and RANKL
BioSample SAMN32860033
SRA SRX19133536

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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