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Sample GSM697315 Query DataSets for GSM697315
Status Public on Aug 01, 2011
Title Control 1040
Sample type RNA
 
Source name laser capture CA1 tissue gray matter from formalin fixed, paraffin embedded specimens
Organism Homo sapiens
Characteristics Sex: Male
age: 87 years old
disease status: Control
Extracted molecule total RNA
Extraction protocol RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion)
Label biotin
Label protocol 50 ng of extracted purified nucleic acid underwent RNA amplification using WT-Ovation FFPE System (NuGen) followed by FL-Ovation cDNA Biotin Module V2 (NuGen) for labeling and microarray (Affymetrix HGU133 v2) hybridization.
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Control; correlation of the expression of each gene was tested with MiniMental Status Examination (MMSE) and neurofibrillary tangle (NFT) scores
Data processing The data were analyzed with Expression Console version 1.1 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Mar 24, 2011
Last update date Jun 26, 2019
Contact name Eric M Blalock
E-mail(s) eric.blalock@uky.edu
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
 
Platform ID GPL570
Series (1)
GSE28146 Microarray analyses of laser-captured hippocampus reveal distinct gray and white matter signatures associated with incipient Alzheimer’s disease
Relations
Affiliated with GSM21232
Reanalyzed by GSE133349

Data table header descriptions
ID_REF Probe Set
VALUE MAS5-calculated Signal intensity
PROBABILITY the probability that a probe set is absent, ranging from 0 to 1 (in this study, probe set with p < 0.05 were considered present)

Data table
ID_REF VALUE PROBABILITY
215306_at 444.1 0.023926
214927_at 1107.4 0.000244
211606_at 5.3 0.954346
212678_at 292.5 0.005859
213929_at 126.8 0.067139
213567_at 1349.4 0.00415
217011_at 10.8 0.981445
217483_at 288.7 0.000732
215769_at 4.7 0.850342
222271_at 144 0.171387
217536_x_at 590.4 0.001953
216539_at 47.8 0.665527
216158_at 850 0.018555
216394_x_at 41.9 0.72583
217136_at 100.4 0.19458
214722_at 7359 0.000244
216965_x_at 523.3 0.00415
212017_at 448.8 0.095215
213392_at 2361 0.000244
217636_at 146.7 0.067627

Total number of rows: 54675

Table truncated, full table size 1345 Kbytes.




Supplementary file Size Download File type/resource
GSM697315.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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