NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM697320 Query DataSets for GSM697320
Status Public on Aug 01, 2011
Title Incipient 1029
Sample type RNA
 
Source name laser capture CA1 tissue gray matter from formalin fixed, paraffin embedded specimens
Organism Homo sapiens
Characteristics Sex: Female
age: 91 years old
disease status: Incipient
Extracted molecule total RNA
Extraction protocol RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion)
Label biotin
Label protocol 50 ng of extracted purified nucleic acid underwent RNA amplification using WT-Ovation FFPE System (NuGen) followed by FL-Ovation cDNA Biotin Module V2 (NuGen) for labeling and microarray (Affymetrix HGU133 v2) hybridization.
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Incipient AD; correlation of the expression of each gene was tested with MiniMental Status Examination (MMSE) and neurofibrillary tangle (NFT) scores
Data processing The data were analyzed with Expression Console version 1.1 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Mar 24, 2011
Last update date Jun 26, 2019
Contact name Eric M Blalock
E-mail(s) eric.blalock@uky.edu
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
 
Platform ID GPL570
Series (1)
GSE28146 Microarray analyses of laser-captured hippocampus reveal distinct gray and white matter signatures associated with incipient Alzheimer’s disease
Relations
Affiliated with GSM21225
Reanalyzed by GSE133349

Data table header descriptions
ID_REF Probe Set
VALUE MAS5-calculated Signal intensity
PROBABILITY the probability that a probe set is absent, ranging from 0 to 1 (in this study, probe set with p < 0.05 were considered present)

Data table
ID_REF VALUE PROBABILITY
215306_at 196.9 0.000244
214927_at 842 0.005859
211606_at 12.3 0.80542
212678_at 160.7 0.037598
213929_at 175.8 0.030273
213567_at 1174.7 0.005859
217011_at 120.4 0.303711
217483_at 927.7 0.000732
215769_at 75.8 0.432373
222271_at 42.7 0.633789
217536_x_at 1515.7 0.000244
216539_at 2.9 0.991943
216158_at 631.4 0.000732
216394_x_at 38.8 0.72583
217136_at 186 0.067627
214722_at 2384.8 0.001953
216965_x_at 453.3 0.000732
212017_at 928.9 0.095215
213392_at 2858.7 0.000244
217636_at 116.8 0.080566

Total number of rows: 54675

Table truncated, full table size 1346 Kbytes.




Supplementary file Size Download File type/resource
GSM697320.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap