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Sample GSM697333 Query DataSets for GSM697333
Status Public on Aug 01, 2011
Title Severe 807
Sample type RNA
Source name laser capture CA1 tissue gray matter from formalin fixed, paraffin embedded specimens
Organism Homo sapiens
Characteristics Sex: Male
age: 93 years old
disease status: Severe
Extracted molecule total RNA
Extraction protocol RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion)
Label biotin
Label protocol 50 ng of extracted purified nucleic acid underwent RNA amplification using WT-Ovation FFPE System (NuGen) followed by FL-Ovation cDNA Biotin Module V2 (NuGen) for labeling and microarray (Affymetrix HGU133 v2) hybridization.
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Severe AD; correlation of the expression of each gene was tested with MiniMental Status Examination (MMSE) and neurofibrillary tangle (NFT) scores
Data processing The data were analyzed with Expression Console version 1.1 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Submission date Mar 24, 2011
Last update date Jun 26, 2019
Contact name Eric M Blalock
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
Platform ID GPL570
Series (1)
GSE28146 Microarray analyses of laser-captured hippocampus reveal distinct gray and white matter signatures associated with incipient Alzheimer’s disease
Affiliated with GSM21207
Reanalyzed by GSE133349

Data table header descriptions
ID_REF Probe Set
VALUE MAS5-calculated Signal intensity
PROBABILITY the probability that a probe set is absent, ranging from 0 to 1 (in this study, probe set with p < 0.05 were considered present)

Data table
215306_at 669.4 0.01416
214927_at 1266.8 0.001953
211606_at 8.3 0.941406
212678_at 348.9 0.080566
213929_at 295.4 0.030273
213567_at 987.1 0.000244
217011_at 229.7 0.5
217483_at 764 0.005859
215769_at 31 0.72583
222271_at 499.5 0.149658
217536_x_at 1944.4 0.001221
216539_at 4.9 0.919434
216158_at 875.3 0.010742
216394_x_at 35.7 0.780518
217136_at 56.8 0.366211
214722_at 3387 0.018555
216965_x_at 695.7 0.01001
212017_at 787.5 0.171387
213392_at 2780.4 0.000244
217636_at 375.6 0.067627

Total number of rows: 54675

Table truncated, full table size 1346 Kbytes.

Supplementary file Size Download File type/resource
GSM697333.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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