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Sample GSM699139 Query DataSets for GSM699139
Status Public on Feb 06, 2012
Title human urine sediment, control subject 5
Sample type RNA
 
Source name human
Organism Homo sapiens
Characteristics gender: Female
age: 75
disease state: normal
Extracted molecule total RNA
Extraction protocol Urine (40-100 ml) was immediately placed on ice, transported to the laboratory and centrifuged at -4C for 5 minutes. Pellets were washed twice with ice-cold phosphate-buffered saline, suspended in 0.8 ml TRIzol and RNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol RNA was stored at -80oC until all samples had been collected, then analyzed at our Microarray Core Facility using the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay protocol.
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Data processing Probe level data were produced by Partek GS (6.10) using the RMA output option. Probe set signal intensities < 4.2 were considered absent, and if > 10 chips rated a probe set absent, that probe set was not considered for further analysis.
 
Submission date Mar 29, 2011
Last update date Feb 07, 2012
Contact name Eric M Blalock
E-mail(s) eric.blalock@uky.edu
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
 
Platform ID GPL6244
Series (1)
GSE28242 Gene expression analysis of urine sediment: evaluation for potential noninvasive markers of interstitial cystitis/bladder pain syndrome

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
7896736 5.754266
7896738 3.18532
7896740 2.943408
7896742 9.28674
7896744 7.769875
7896746 8.472357
7896748 7.883534
7896750 2.821138
7896752 8.067058
7896754 5.574138
7896756 5.045781
7896759 5.783064
7896761 6.50996
7896779 6.564027
7896798 7.071332
7896817 8.011457
7896822 7.783111
7896859 5.718442
7896861 3.503772
7896863 6.256671

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM699139.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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