|
Status |
Public on Feb 06, 2012 |
Title |
human urine sediment, without lesions subject 4 |
Sample type |
RNA |
|
|
Source name |
human
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 60 disease state: PBS without lesions
|
Extracted molecule |
total RNA |
Extraction protocol |
Urine (40-100 ml) was immediately placed on ice, transported to the laboratory and centrifuged at -4C for 5 minutes. Pellets were washed twice with ice-cold phosphate-buffered saline, suspended in 0.8 ml TRIzol and RNA was extracted according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
RNA was stored at -80oC until all samples had been collected, then analyzed at our Microarray Core Facility using the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay protocol.
|
|
|
Hybridization protocol |
Standard Affymetrix
|
Scan protocol |
Standard Affymetrix
|
Data processing |
Probe level data were produced by Partek GS (6.10) using the RMA output option. Probe set signal intensities < 4.2 were considered absent, and if > 10 chips rated a probe set absent, that probe set was not considered for further analysis.
|
|
|
Submission date |
Mar 29, 2011 |
Last update date |
Feb 07, 2012 |
Contact name |
Eric M Blalock |
E-mail(s) |
eric.blalock@uky.edu
|
Phone |
859-323-8033
|
Organization name |
University of Kentucky
|
Department |
Molecular and Biomedical Pharmacology
|
Lab |
Blalock
|
Street address |
800 Rose St.
|
City |
Lexington |
State/province |
KY |
ZIP/Postal code |
40475 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE28242 |
Gene expression analysis of urine sediment: evaluation for potential noninvasive markers of interstitial cystitis/bladder pain syndrome |
|