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Sample GSM699143 Query DataSets for GSM699143
Status Public on Feb 06, 2012
Title human urine sediment, without lesions subject 4
Sample type RNA
 
Source name human
Organism Homo sapiens
Characteristics gender: Female
age: 60
disease state: PBS without lesions
Extracted molecule total RNA
Extraction protocol Urine (40-100 ml) was immediately placed on ice, transported to the laboratory and centrifuged at -4C for 5 minutes. Pellets were washed twice with ice-cold phosphate-buffered saline, suspended in 0.8 ml TRIzol and RNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol RNA was stored at -80oC until all samples had been collected, then analyzed at our Microarray Core Facility using the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay protocol.
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Data processing Probe level data were produced by Partek GS (6.10) using the RMA output option. Probe set signal intensities < 4.2 were considered absent, and if > 10 chips rated a probe set absent, that probe set was not considered for further analysis.
 
Submission date Mar 29, 2011
Last update date Feb 07, 2012
Contact name Eric M Blalock
E-mail(s) eric.blalock@uky.edu
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
 
Platform ID GPL6244
Series (1)
GSE28242 Gene expression analysis of urine sediment: evaluation for potential noninvasive markers of interstitial cystitis/bladder pain syndrome

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
7896736 5.292299
7896738 3.396494
7896740 3.649018
7896742 6.239594
7896744 5.67927
7896746 9.292497
7896748 7.752398
7896750 3.502069
7896752 8.294275
7896754 4.811042
7896756 5.263417
7896759 6.304613
7896761 6.850462
7896779 7.254805
7896798 7.614685
7896817 7.701448
7896822 8.371329
7896859 6.425487
7896861 4.274273
7896863 6.858591

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM699143.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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