|
| Status |
Public on Feb 06, 2012 |
| Title |
human urine sediment, with lesions subject 1 |
| Sample type |
RNA |
| |
|
| Source name |
human
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 82
disease state: PBS with lesions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Urine (40-100 ml) was immediately placed on ice, transported to the laboratory and centrifuged at -4C for 5 minutes. Pellets were washed twice with ice-cold phosphate-buffered saline, suspended in 0.8 ml TRIzol and RNA was extracted according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was stored at -80oC until all samples had been collected, then analyzed at our Microarray Core Facility using the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Data processing |
Probe level data were produced by Partek GS (6.10) using the RMA output option. Probe set signal intensities < 4.2 were considered absent, and if > 10 chips rated a probe set absent, that probe set was not considered for further analysis.
|
| |
|
| Submission date |
Mar 29, 2011 |
| Last update date |
Feb 07, 2012 |
| Contact name |
Eric M Blalock |
| E-mail(s) |
eric.blalock@uky.edu
|
| Phone |
859-323-8033
|
| Organization name |
University of Kentucky
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Blalock
|
| Street address |
800 Rose St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40475 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE28242 |
Gene expression analysis of urine sediment: evaluation for potential noninvasive markers of interstitial cystitis/bladder pain syndrome |
|
