|
| Status |
Public on Aug 29, 2011 |
| Title |
prostate cancer sample 7 [gene expr] |
| Sample type |
RNA |
| |
|
| Source name |
prostate cancer_recurrence, castration-resistant
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Castration-resistant prostate cancer
treatment: Casodex, Suprefact
tissue: prostate cancer tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using either TRIzol (Invitrogen, Carlsbad, CA) or LiCl / urea isolation–based methods.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 3 micrograms of mRNA per sample according to manufacturers (Affymetrix) instructions.
|
| |
|
| Hybridization protocol |
15 micrograms of fragmented cRNA from each sample was used for hybridizations. The arrays were stained with phycoerythrin-streptavidin and the signal intensity was amplified by treatment with a biotin-conjugated anti-streptavidin antibody, followed by a second staining using phycoerythrin-streptavidin.
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 (Affymetrix)
|
| Data processing |
Basic GeneChip array analysis was performed for the obtained image file. Data preprocessing was done using the RMA algorithm implemented in the Bioconductor package “affy.
|
| |
|
| Submission date |
Apr 05, 2011 |
| Last update date |
Aug 29, 2011 |
| Contact name |
Maija K Wolf |
| E-mail(s) |
maija.wolf@helsinki.fi
|
| Organization name |
Institute for Molecular Medicine Finland (FIMM)
|
| Street address |
Biomedicum Helsinki 2U, Tukholmankatu 8,
|
| City |
Helsinki |
| ZIP/Postal code |
FIN-00290 |
| Country |
Finland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28403 |
Integrative genomic, transcriptomic and RNAi analysis indicates a potential oncogenic role for FAM110B in castration-resistant prostate cancer. |
|
