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Sample GSM701962 Query DataSets for GSM701962
Status Public on Aug 29, 2011
Title prostate cancer sample 15 [gene expr]
Sample type RNA
 
Source name prostate cancer_lymph node metastasis
Organism Homo sapiens
Characteristics disease state: Advanced prostate cancer
treatment: None
tissue: lymph node metastatic tumor
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using either TRIzol (Invitrogen, Carlsbad, CA) or LiCl / urea isolation–based methods.
Label biotin
Label protocol Biotinylated cRNA was prepared from 3 micrograms of mRNA per sample according to manufacturers (Affymetrix) instructions.
 
Hybridization protocol 15 micrograms of fragmented cRNA from each sample was used for hybridizations. The arrays were stained with phycoerythrin-streptavidin and the signal intensity was amplified by treatment with a biotin-conjugated anti-streptavidin antibody, followed by a second staining using phycoerythrin-streptavidin.
Scan protocol Arrays were scanned using the GeneChip Scanner 3000 (Affymetrix)
Data processing Basic GeneChip array analysis was performed for the obtained image file. Data preprocessing was done using the RMA algorithm implemented in the Bioconductor package “affy.
 
Submission date Apr 05, 2011
Last update date Aug 29, 2011
Contact name Maija K Wolf
E-mail(s) maija.wolf@helsinki.fi
Organization name Institute for Molecular Medicine Finland (FIMM)
Street address Biomedicum Helsinki 2U, Tukholmankatu 8,
City Helsinki
ZIP/Postal code FIN-00290
Country Finland
 
Platform ID GPL570
Series (1)
GSE28403 Integrative genomic, transcriptomic and RNAi analysis indicates a potential oncogenic role for FAM110B in castration-resistant prostate cancer.

Data table header descriptions
ID_REF
VALUE Log 2 RMA signal

Data table
ID_REF VALUE
1007_s_at 9.93896156774658
1053_at 6.5430933374876
117_at 4.75858924043201
121_at 6.8169073227205
1255_g_at 2.88086134881524
1294_at 7.28538652713309
1316_at 4.49064071992884
1320_at 3.92003603592533
1405_i_at 5.81425221933938
1431_at 3.36086421067193
1438_at 5.8907063309935
1487_at 6.77028469012459
1494_f_at 4.61888805036489
1552256_a_at 7.97327279496627
1552257_a_at 8.50275068894494
1552258_at 3.23207891585432
1552261_at 3.70794061668702
1552263_at 4.77017061741669
1552264_a_at 7.69604634751002
1552266_at 2.77818999573218

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM701962_15.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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