NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM7031937 Query DataSets for GSM7031937
Status Public on Feb 07, 2023
Title Chordoma 10
Sample type RNA
 
Source name Chordoma Tumor
Organism Homo sapiens
Characteristics tissue: Chordoma Tumor
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 875.37658616204
1053_at 220.794140404816
117_at 4023.84746305257
121_at 397.170333295759
1255_g_at 14.7713217694617
1294_at 2172.37921979849
1316_at 1219.2450813403
1320_at 568.487361931824
1405_i_at 840.344474847178
1431_at 69.1756446729336
1438_at 24.3539204269395
1487_at 248.350850340102
1494_f_at 280.071494716051
1552256_a_at 71.0655637866775
1552257_a_at 139.983260836087
1552258_at 299.267130691044
1552261_at 25.2955911518088
1552263_at 238.625872166696
1552264_a_at 189.180696237532
1552266_at 100.50398517802

Total number of rows: 54675

Table truncated, full table size 1286 Kbytes.




Supplementary file Size Download File type/resource
GSM7031937_Ch10_1590G.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap