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Sample GSM7031939 Query DataSets for GSM7031939
Status Public on Feb 07, 2023
Title CH22-3
Sample type RNA
 
Source name CH22 cell line
Organism Homo sapiens
Characteristics cell line: CH22
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 1533.70380804632
1053_at 1550.63461986029
117_at 25.306413843581
121_at 112.885685921007
1255_g_at 2.61231102257208
1294_at 86.6807575004542
1316_at 131.367957169262
1320_at 11.8398423612366
1405_i_at 131.827567095349
1431_at 33.4727141348555
1438_at 7.62291197482964
1487_at 784.484497432256
1494_f_at 16.0764375662222
1552256_a_at 615.415265156101
1552257_a_at 3640.0321941634
1552258_at 76.2779640564863
1552261_at 25.6273159602191
1552263_at 416.433805573776
1552264_a_at 222.154930674708
1552266_at 20.1591701454083

Total number of rows: 54675

Table truncated, full table size 1287 Kbytes.




Supplementary file Size Download File type/resource
GSM7031939_CH22-3_B.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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