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Sample GSM7031941 Query DataSets for GSM7031941
Status Public on Feb 07, 2023
Title Chordoma 4
Sample type RNA
 
Source name Chordoma Tumor
Organism Homo sapiens
Characteristics tissue: Chordoma Tumor
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 2176.87028582693
1053_at 246.400408441598
117_at 978.706400568448
121_at 154.803006941018
1255_g_at 29.6348547825232
1294_at 785.400509940497
1316_at 307.507536436789
1320_at 152.071020839301
1405_i_at 3188.11849473848
1431_at 214.335281013225
1438_at 4.7658732028138
1487_at 256.490644044905
1494_f_at 6.26784950967161
1552256_a_at 117.849716322784
1552257_a_at 232.098018464582
1552258_at 125.800540162476
1552261_at 96.9764960474313
1552263_at 248.276764260361
1552264_a_at 179.021260123813
1552266_at 11.2572520169412

Total number of rows: 54675

Table truncated, full table size 1286 Kbytes.




Supplementary file Size Download File type/resource
GSM7031941_Ch4_1590B.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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