|
| Status |
Public on Feb 07, 2023 |
| Title |
Fetal Spine |
| Sample type |
RNA |
| |
|
| Source name |
Fetal Spin Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Fetal Spinal Tissue
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000
|
| Data processing |
CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
|
| |
|
| Submission date |
Feb 07, 2023 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Stefan Halvorsen |
| E-mail(s) |
shalvorsen@mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Computational and Integrative Biology
|
| Lab |
Seed Lab
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE205458 |
Expression data from chordoma and notochord |
| GSE224776 |
Expression data from chordoma and notochord (microarray) |
|
