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Sample GSM7031943 Query DataSets for GSM7031943
Status Public on Feb 07, 2023
Title Fetal Spine
Sample type RNA
 
Source name Fetal Spin Tissue
Organism Homo sapiens
Characteristics tissue: Fetal Spinal Tissue
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 440.758327681488
1053_at 347.574682293402
117_at 81.2358760990408
121_at 137.473333962006
1255_g_at 86.9599688454947
1294_at 329.515875890938
1316_at 872.827319692548
1320_at 579.410860263665
1405_i_at 52.3790397485132
1431_at 94.651805160548
1438_at 137.045896719361
1487_at 153.023965328519
1494_f_at 14.5258447977556
1552256_a_at 37.1477928445004
1552257_a_at 390.106608819974
1552258_at 158.0254620736
1552261_at 27.4324001131135
1552263_at 58.5276014470717
1552264_a_at 364.122307923206
1552266_at 67.0219509661928

Total number of rows: 54675

Table truncated, full table size 1286 Kbytes.




Supplementary file Size Download File type/resource
GSM7031943_FetalSpine_1590D.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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