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Sample GSM7031944 Query DataSets for GSM7031944
Status Public on Feb 07, 2023
Title Notochord 1-1
Sample type RNA
 
Source name Notochord
Organism Homo sapiens
Characteristics tissue: Notochord
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 1316.36750158154
1053_at 98.0256826155103
117_at 314.326135613094
121_at 258.940815755483
1255_g_at 59.8072930527849
1294_at 586.965640210521
1316_at 1428.74608673662
1320_at 566.834505691352
1405_i_at 13.1081416575095
1431_at 104.520431240671
1438_at 69.2323760937147
1487_at 373.944838215264
1494_f_at 89.433213805173
1552256_a_at 149.11856913001
1552257_a_at 245.754856285674
1552258_at 327.681870127854
1552261_at 116.603183739247
1552263_at 212.954831422708
1552264_a_at 137.759038029145
1552266_at 75.7948981571981

Total number of rows: 54675

Table truncated, full table size 1286 Kbytes.




Supplementary file Size Download File type/resource
GSM7031944_Noto1-1_1700A.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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