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Sample GSM7031945 Query DataSets for GSM7031945
Status Public on Feb 07, 2023
Title Notochord 1-2
Sample type RNA
 
Source name Notochord
Organism Homo sapiens
Characteristics tissue: Notochord
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 2210.07200970473
1053_at 151.703050963063
117_at 175.288642885978
121_at 225.756137663368
1255_g_at 5.43795135023432
1294_at 724.744927295814
1316_at 673.484644193038
1320_at 762.156866970073
1405_i_at 40.7441066784177
1431_at 8.82856538691706
1438_at 22.3341676466146
1487_at 369.02660645525
1494_f_at 103.710762400004
1552256_a_at 146.771112545544
1552257_a_at 190.216863526208
1552258_at 243.914429879105
1552261_at 23.8471479972
1552263_at 52.6775796527248
1552264_a_at 133.13304220346
1552266_at 110.411060728359

Total number of rows: 54675

Table truncated, full table size 1286 Kbytes.




Supplementary file Size Download File type/resource
GSM7031945_Noto1-2_1700B.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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