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Sample GSM7031946 Query DataSets for GSM7031946
Status Public on Feb 07, 2023
Title Notochord 2
Sample type RNA
 
Source name Notochord
Organism Homo sapiens
Characteristics tissue: Notochord
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 2968.89340109359
1053_at 71.3014418370942
117_at 353.104508072152
121_at 184.05447622704
1255_g_at 7.68273344631843
1294_at 437.317362440406
1316_at 1673.92036373225
1320_at 254.020429204074
1405_i_at 11.9530318350254
1431_at 185.690154666958
1438_at 215.001430555229
1487_at 43.6659088989317
1494_f_at 233.611205606366
1552256_a_at 345.460144463564
1552257_a_at 387.925574875615
1552258_at 310.421753115767
1552261_at 258.619790352849
1552263_at 168.450025405581
1552264_a_at 340.010195574554
1552266_at 60.9261146155701

Total number of rows: 54675

Table truncated, full table size 1286 Kbytes.




Supplementary file Size Download File type/resource
GSM7031946_Noto2_1700C.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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