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Sample GSM7031947 Query DataSets for GSM7031947
Status Public on Feb 07, 2023
Title UCH1
Sample type RNA
 
Source name UCH1 cell line
Organism Homo sapiens
Characteristics cell line: UCH1
Treatment protocol None
Growth protocol None
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Notochord samples were prepared by laser capture microdissection and RNA extracted using Arcturus PicoPure RNA Isolation kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol cRNA was hybridized on GeneChip Human Genome U133 Plus 2.0 Array according to manufacturer directions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using GeneChip Scanner 3000
Data processing CEL files were read into R using the bioconductor affy package. Expression values were calculated using the mas5 algorithm in the affy package.
 
Submission date Feb 07, 2023
Last update date Feb 07, 2023
Contact name Stefan Halvorsen
E-mail(s) shalvorsen@ccib.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Computational and Integrative Biology
Lab Seed Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL570
Series (2)
GSE205458 Expression data from chordoma and notochord
GSE224776 Expression data from chordoma and notochord (microarray)

Data table header descriptions
ID_REF
VALUE mas5 normalized expression values.

Data table
ID_REF VALUE
1007_s_at 4740.28924101917
1053_at 800.847056334703
117_at 57.498290286227
121_at 61.5595083926224
1255_g_at 2.66130888074609
1294_at 416.310297225579
1316_at 167.272642682919
1320_at 94.1006529702605
1405_i_at 777.169752810037
1431_at 16.992253926462
1438_at 4.8134012338695
1487_at 752.991214277328
1494_f_at 3.02346846899778
1552256_a_at 198.969571469783
1552257_a_at 1154.30241487722
1552258_at 78.2457968101268
1552261_at 2.82493729134916
1552263_at 205.710222749962
1552264_a_at 71.8607681130142
1552266_at 24.9400042768954

Total number of rows: 54675

Table truncated, full table size 1287 Kbytes.




Supplementary file Size Download File type/resource
GSM7031947_UCH1_C.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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