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Sample GSM703871 Query DataSets for GSM703871
Status Public on Jan 23, 2012
Title Monocytes rep1 miRNA (Roche)
Sample type RNA
Source name CD14+ monocytes from healthy donor pool 1
Organism Homo sapiens
Characteristics cell type: Monocytes
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description CD14+ monocytes, CD8+ T cells, CD56+ NK cells and CD19 B cells were isolated using the Human CD14 positive Selection Kit, Human CD8+ T cells Enrichment Kit, EasySep® Human CD56+ NK cells Enrichment Kit, EasySep® Human CD19+ B cells Enrichment Kit (all from Stem Cell Technologies) respectively according to manufacturer’s instructions.
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
VALUE log2 RMA signal intensity

Data table
14q-0_st 0.844915
14qI-1_st 2.08338
14qI-2_st 0.842399
14qI-3_x_st 1.15345
14qI-4_st 1.20039
14qI-4_x_st 1.25085
14qI-5_st 1.40879
14qI-6_st 0.699218
14qI-6_x_st 0.928099
14qI-7_st 0.885709
14qI-8_st 0.845003
14qI-8_x_st 1.08065
14qI-9_x_st 1.44293
14qII-10_st 1.05491
14qII-11_st 0.948808
14qII-11_x_st 1.20102
14qII-12_st 0.718201
14qII-12_x_st 1.20421
14qII-13_st 1.26156
14qII-14_st 1.19418

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.

Supplementary file Size Download File type/resource
GSM703871_miRNA_CD14_EXP1.CEL.gz 167.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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