GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM703883 Query DataSets for GSM703883
Status Public on Jan 23, 2012
Title B cells rep4 miRNA (Roche)
Sample type RNA
Source name CD19+ B cells from healthy donor pool 8
Organism Homo sapiens
Characteristics cell type: B cells
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description CD14+ monocytes, CD8+ T cells, CD56+ NK cells and CD19 B cells were isolated using the Human CD14 positive Selection Kit, Human CD8+ T cells Enrichment Kit, EasySep® Human CD56+ NK cells Enrichment Kit, EasySep® Human CD19+ B cells Enrichment Kit (all from Stem Cell Technologies) respectively according to manufacturer’s instructions.
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
VALUE log2 RMA signal intensity

Data table
14q-0_st 1.01756
14qI-1_st 2.02387
14qI-2_st 0.756282
14qI-3_x_st 1.46827
14qI-4_st 1.43325
14qI-4_x_st 1.80989
14qI-5_st 1.08061
14qI-6_st 1.75527
14qI-6_x_st 0.80411
14qI-7_st 1.32433
14qI-8_st 1.28159
14qI-8_x_st 1.28606
14qI-9_x_st 1.05956
14qII-10_st 1.00719
14qII-11_st 1.00958
14qII-11_x_st 0.847216
14qII-12_st 0.946981
14qII-12_x_st 1.19043
14qII-13_st 1.53713
14qII-14_st 1.30069

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.

Supplementary file Size Download File type/resource
GSM703883_miRNA_CD19_EXP8.CEL.gz 148.6 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap