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Sample GSM703888 Query DataSets for GSM703888
Status Public on Jan 23, 2012
Title CD4+ T cells rep4 miRNA (Roche)
Sample type RNA
Source name CD4+ T cells from healthy donor pool 8
Organism Homo sapiens
Characteristics cell type: CD4+ T cells
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description CD4+ T cells were isolated from the monocytes-depleted fraction (obtained during the monocytes isolation) using the EasySep® Human CD4 Positive Selection Kit (Stemcell Technologies) according to manufacturer’s instructions.
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
VALUE log2 RMA signal intensity

Data table
14q-0_st 1.04569
14qI-1_st 1.68278
14qI-2_st 0.811927
14qI-3_x_st 1.1811
14qI-4_st 1.54401
14qI-4_x_st 1.21015
14qI-5_st 1.09168
14qI-6_st 1.00143
14qI-6_x_st 1.56581
14qI-7_st 0.913492
14qI-8_st 0.78709
14qI-8_x_st 1.15799
14qI-9_x_st 1.43455
14qII-10_st 1.70347
14qII-11_st 0.841325
14qII-11_x_st 1.50154
14qII-12_st 0.906433
14qII-12_x_st 1.12371
14qII-13_st 1.43896
14qII-14_st 0.808107

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.

Supplementary file Size Download File type/resource
GSM703888_miRNA_CD4_EXP8.CEL.gz 153.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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