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Sample GSM703895 Query DataSets for GSM703895
Status Public on Jan 23, 2012
Title CD8+ T cells rep2 miRNA (Roche)
Sample type RNA
 
Source name CD8+ T cells from healthy donor pool 11
Organism Homo sapiens
Characteristics cell type: CD8+ T cells
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
 
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description CD14+ monocytes, CD8+ T cells, CD56+ NK cells and CD19 B cells were isolated using the Human CD14 positive Selection Kit, Human CD8+ T cells Enrichment Kit, EasySep® Human CD56+ NK cells Enrichment Kit, EasySep® Human CD19+ B cells Enrichment Kit (all from Stem Cell Technologies) respectively according to manufacturer’s instructions.
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
 
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
E-mail(s) donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 0.818999
14qI-1_st 1.59887
14qI-2_st 0.902725
14qI-3_x_st 0.975809
14qI-4_st 1.00876
14qI-4_x_st 1.37522
14qI-5_st 1.21358
14qI-6_st 1.00515
14qI-6_x_st 1.11597
14qI-7_st 1.22902
14qI-8_st 1.08247
14qI-8_x_st 1.18394
14qI-9_x_st 1.23858
14qII-10_st 1.08776
14qII-11_st 1.02727
14qII-11_x_st 1.27798
14qII-12_st 1.24681
14qII-12_x_st 1.18238
14qII-13_st 1.24995
14qII-14_st 0.981518

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.




Supplementary file Size Download File type/resource
GSM703895_miRNA_CD8_EXP11.CEL.gz 156.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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