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Sample GSM703901 Query DataSets for GSM703901
Status Public on Jan 23, 2012
Title Eosinophils rep3 miRNA (Roche)
Sample type RNA
Source name Eosinophils from healthy donor pool 7
Organism Homo sapiens
Characteristics cell type: Eosinophils
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description Isolated using EasySep® Human Eosinophil Enrichment Kit (Stem Cell Technologies)
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
VALUE log2 RMA signal intensity

Data table
14q-0_st 1.05814
14qI-1_st 2.08827
14qI-2_st 0.929602
14qI-3_x_st 1.27999
14qI-4_st 1.36377
14qI-4_x_st 1.60333
14qI-5_st 1.5564
14qI-6_st 0.933717
14qI-6_x_st 1.1626
14qI-7_st 1.09655
14qI-8_st 1.39211
14qI-8_x_st 0.932084
14qI-9_x_st 1.17373
14qII-10_st 1.18212
14qII-11_st 0.781215
14qII-11_x_st 1.24904
14qII-12_st 0.841159
14qII-12_x_st 0.771731
14qII-13_st 1.07606
14qII-14_st 0.700276

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.

Supplementary file Size Download File type/resource
GSM703901_miRNA_EOSINO_EXP7.CEL.gz 153.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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