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Sample GSM703903 Query DataSets for GSM703903
Status Public on Jan 23, 2012
Title mDC rep1 miRNA (Roche)
Sample type RNA
 
Source name mDCs from healthy donor pool 14
Organism Homo sapiens
Characteristics cell type: mDCs
Extracted molecule total RNA
Extraction protocol Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
Label Biotin
Label protocol Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
 
Hybridization protocol Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
Scan protocol GeneChips were scanned using the GC 30007G scanner.
Description CD123+ plasmacytoid dendritic cells (pDCs), CD11c+ myeloid dendritic cells (mDCs) and CD14+ monocytes were isolated using the EasySep® Human pDCs Enrichment Kit, a custom EasySep® Human mDCs Enrichment Kit (containing antibodies directed against CD3, CD19, CD14, CD16, CD19, CD25, CD34, CD56, CD66b, GlyA, CD85g, CD45RA, CD123 and dextran) and the Human CD14 positive Selection Kit (all from Stemcell Biotechnologies) respectively according to manufacturer’s instructions.
Data processing Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
 
Submission date Apr 08, 2011
Last update date Jan 24, 2012
Contact name Donavan Cheng
E-mail(s) donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL8786
Series (2)
GSE28487 miRNA expression profiling of human immune cell subsets (Roche)
GSE28492 miRNA and mRNA expression profiling of human immune cell subsets

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
14q-0_st 1.02893
14qI-1_st 3.22874
14qI-2_st 1.35576
14qI-3_x_st 1.98069
14qI-4_st 1.2972
14qI-4_x_st 2.22827
14qI-5_st 1.81732
14qI-6_st 1.09906
14qI-6_x_st 1.50585
14qI-7_st 2.06174
14qI-8_st 1.2972
14qI-8_x_st 1.36218
14qI-9_x_st 1.18744
14qII-10_st 1.1581
14qII-11_st 1.11459
14qII-11_x_st 1.2972
14qII-12_st 0.748618
14qII-12_x_st 1.43126
14qII-13_st 1.58511
14qII-14_st 0.742576

Total number of rows: 7815

Table truncated, full table size 183 Kbytes.




Supplementary file Size Download File type/resource
GSM703903_miRNA_mDC_EXP14.CEL.gz 138.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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