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Sample GSM7048217 Query DataSets for GSM7048217
Status Public on Jun 09, 2023
Title CH-Head_1
Sample type SRA
 
Source name head skin
Organism Sus scrofa
Characteristics breed: Chenghua
tissue: head skin
cell type: Skin cells
Sex: female
age: 180 days old
Extracted molecule total RNA
Extraction protocol Single-cell transcriptome analyze from three CH pigs at six different anatomical body areas (head, ear, shoulder, back, abdomen, leg) and three LW pigs with one region (back).The fresh skin samples were thoroughly scraped off the hair and subcutaneous fat and were washed thrice with ice-cold Dulbecco’s Phosphate-Buffered Salline (1xDPBS).
Single-cell suspension were subjected to a series of progress, including droplet encapsulation, emulsion breakage, mRNA captured bead collection, reverse transcription, and cDNA amplification and purification, to generate barcoded libraries
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model DNBSEQ-G400
 
Description Skin, replicate 1
Data processing Cells genes were expressed in less than three cells, and cells were removed on the basis of detected genes number with a minimal of 200. Mitochondrial gene expression was set at a threshold of 5% for per cell
Assembly: Sus scrofa11.1
Supplementary files format and content: Tab-separated values files and matrix files
 
Submission date Feb 16, 2023
Last update date Jun 09, 2023
Contact name Qin Zou
E-mail(s) zouqin182023@126.com
Organization name Sichuan Agricultural University
Street address No.211, Huimin Road
City Chengdu
ZIP/Postal code 611130
Country China
 
Platform ID GPL29847
Series (1)
GSE225416 A single-cell transcriptome atlas of pig skin characterizes anatomic positional heterogeneity
Relations
BioSample SAMN33325486
SRA SRX19391819

Supplementary file Size Download File type/resource
GSM7048217_Head_1_barcodes.tsv.gz 25.8 Kb (ftp)(http) TSV
GSM7048217_Head_1_features.tsv.gz 151.8 Kb (ftp)(http) TSV
GSM7048217_Head_1_matrix.mtx.gz 36.5 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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