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Sample GSM7048308 Query DataSets for GSM7048308
Status Public on Feb 29, 2024
Title interscapular brown adipose tissue, 10day cold exposed, Mafbf/f-LysMcre 3
Sample type SRA
Source name interscapular brown adipose tissue
Organism Mus musculus
Characteristics tissue: interscapular brown adipose tissue
strain: C57BL/6J
cell type: tissue
genotype: Mafbf/f-LysMcre
treatment: 10 days, 4 degree cold exposure
Extracted molecule total RNA
Extraction protocol RNA was harvested using Rneasy mini kit (Qiagen). 500ng of total RNA was used for the construction of sequencing libraries.
Library construction and RNA seq peformed by University of Tsukuba, RNA seq service
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description Single bam file generated for each sample using STAR, from paired-end two separate fastq files
Data processing STAR version=STAR_2.6.1a_08-27
Sequence read were alligned and mapped using default paramaters of STAR version STAR_2.6.1a_08-27
Read count performed by STAR
Normalization and DEG analysis done by edgeR tool
Assembly: mm10
Supplementary files format and content: tab-delimited text files include raw count and TPM values for each Sample
Submission date Feb 16, 2023
Last update date Feb 29, 2024
Contact name Michito Hamada
Phone 029-853-7516
Organization name University of Tsukuba
Department Department of Anatomy and Embryology
Lab Takahashi Lab
Street address 1-1-1 Tennodai,
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8575
Country Japan
Platform ID GPL19057
Series (1)
GSE225421 Macrophage specific Mafb deficiency effects brown adipose tissue's cold adaptability
BioSample SAMN33325551
SRA SRX19391489

Supplementary file Size Download File type/resource
GSM7048308_Mafbff_LysMCre_03.txt.gz 208.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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