|
| Status |
Public on Oct 14, 2023 |
| Title |
HCT116, VSNL1 siRNA |
| Sample type |
RNA |
| |
|
| Source name |
Colorectal cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
knock down: siVSNL1
cell line: HCT116
|
| Growth protocol |
All cell lines were cultured in accordance with the supplier's instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA for expression analysis was extracted using an RNeasy Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA targets were generated using Quick Amp Labeling Kit, one-color (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Gene Expression Hybridization kit was used for hybridization in accordance with the manufacture's instructions (Agilent Technologies).
|
| Scan protocol |
Microarray images were obtained by using a laser confocal scanner G2565BA (Agilent Technologies).
|
| Data processing |
Feature Extraction software (Version 9.1, Agilent Technologies) with the manufacturer’s recommended settings was applied for the microarray image analysis. Analysis of the microarray images was performed with Genespring 7.3.1 Software (Agilent Technologies).
|
| |
|
| Submission date |
Feb 21, 2023 |
| Last update date |
Oct 14, 2023 |
| Contact name |
Naoki Hijiya |
| E-mail(s) |
hijiya@oita-u.ac.jp
|
| Phone |
1975865693
|
| Organization name |
Oita University
|
| Department |
Medicine
|
| Lab |
Molecular pathology
|
| Street address |
1-1, Idaigaoka Hasama-machi
|
| City |
Yufu |
| State/province |
Oita |
| ZIP/Postal code |
879-5593 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13497 |
| Series (1) |
| GSE225763 |
Identification of genes regulated by VSNL1 in colorectal cancer cells |
|
