|
Status |
Public on Mar 25, 2024 |
Title |
MB_SHHb_T35 |
Sample type |
genomic |
|
|
Source name |
tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue: medulloblastoma cell line: --
|
Treatment protocol |
No treatment
|
Growth protocol |
Cell lines are cultured in 2D or 3D cultures and in serum- or free serum- media.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted with phenol- chloroform and precipitated with isopropanol.
|
Label |
Cy3 and Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation EPIC Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using iScan Platform using standard recommended Illumina setting
|
Description |
DNA from tumor tissue medulloblastoma_35 formalin-fixed
|
Data processing |
GenomeStudio software v2.0
|
|
|
Submission date |
Feb 22, 2023 |
Last update date |
Mar 25, 2024 |
Contact name |
Evelina Miele |
E-mail(s) |
evelina.miele@opbg.net
|
Organization name |
Ospedale Pediatrico Bambino Gesù
|
Street address |
Viale Baldelli 38
|
City |
Rome |
ZIP/Postal code |
00146 |
Country |
Italy |
|
|
Platform ID |
GPL23976 |
Series (1) |
GSE225810 |
Evaluating cell culture reliability in pediatric brain tumor primary cells through DNA methylation profiling |
|