|
| Status |
Public on Jul 15, 2011 |
| Title |
trophectoderm TE_D2E2 |
| Sample type |
RNA |
| |
|
| Source name |
Human mural trophectoderm_D2E2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: mural trophectoderm
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy micro kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) protocol ‘small sample protocol II’, starting from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Human mural trophectoderm was mechanically separated from 5 days blastocyst and lysed for RNA extraction.
|
| Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 09, 2011 |
| Last update date |
Oct 18, 2011 |
| Contact name |
Qiang BAI |
| E-mail(s) |
qiang.bai@inserm.fr
|
| Phone |
+33 (0)4 67 41 52 08
|
| Organization name |
PhyMedExp INSERM U1046
|
| Lab |
Maladies respiratoires et environnement
|
| Street address |
371 Avenue du Doyen Gaston Giraud
|
| City |
Montpellier |
| ZIP/Postal code |
34295 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE33025 |
