|
Status |
Public on Jul 15, 2011 |
Title |
trophectoderm TE_5 |
Sample type |
RNA |
|
|
Source name |
Human mural trophectoderm_tropho_5
|
Organism |
Homo sapiens |
Characteristics |
tissue: mural trophectoderm
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNeasy micro kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer.
|
Label |
Biotin
|
Label protocol |
Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) protocol ‘small sample protocol II’, starting from 100 ng total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Human mural trophectoderm was mechanically separated from 5 days blastocyst and lysed for RNA extraction.
|
Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
|
|
Submission date |
Apr 09, 2011 |
Last update date |
Oct 18, 2011 |
Contact name |
Qiang BAI |
E-mail(s) |
qiang.bai@inserm.fr
|
Phone |
+33 (0)4 67 41 52 08
|
Organization name |
PhyMedExp INSERM U1046
|
Lab |
Maladies respiratoires et environnement
|
Street address |
371 Avenue du Doyen Gaston Giraud
|
City |
Montpellier |
ZIP/Postal code |
34295 |
Country |
France |
|
|
Platform ID |
GPL570 |
Series (1) |
|
Relations |
Reanalyzed by |
GSE33025 |