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Sample GSM7109979 Query DataSets for GSM7109979
Status Public on Mar 14, 2024
Title Xu142_0_rep3
Sample type SRA
Source name ovule
Organism Gossypium hirsutum
Characteristics tissue: ovule
dpa: 0
genotype: Xu142
treatment: Unhandled
Extracted molecule total RNA
Extraction protocol 1 μg of total RNA was used to generate the sequencing libraries, which were sequenced using the Illumina platform by Beijing novogene Co., Ltd. (, Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing The mRNA with polyA tail was enriched by Oligo (dT) magnetic beads.
Fragmentation buffer was added to randomly break the mRNA into short fragments.
The cDNA was synthesized by reverse transcription using short fragments as templates, and then the cDNA ends were repaired, A-tailed, and connected with UMI tags and sequencing connectors
Finally, the library was obtained by PCR amplification
Supplementary files format and content: tab-delimited text files include readcount values for each Sample …
Submission date Mar 21, 2023
Last update date Mar 14, 2024
Contact name YingQiang Long
Organization name School of Agricultural Sciences, Zhengzhou University
Street address Science Avenue
City Science Avenue
ZIP/Postal code 450001
Country China
Platform ID GPL29713
Series (1)
GSE227870 Histone H3 lysine 27 trimethylation (H3K27me3) modulates the fiber initiation in upland cotton
BioSample SAMN33845675
SRA SRX19742264

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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