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Sample GSM7109980

Query DataSets for GSM7109980

Status

Public on Mar 14, 2024

Title

Xu142_1_rep1

Sample type

SRA

Source name

ovule

Organism

Gossypium hirsutum

Characteristics

tissue: ovule
dpa: 1
genotype: Xu142
treatment: Unhandled

Extracted molecule

total RNA

Extraction protocol

1 μg of total RNA was used to generate the sequencing libraries, which were sequenced using the Illumina platform by Beijing novogene Co., Ltd. (www.novogene.com, Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols.

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

Illumina NovaSeq 6000

Data processing

The mRNA with polyA tail was enriched by Oligo (dT) magnetic beads.
Fragmentation buffer was added to randomly break the mRNA into short fragments.
The cDNA was synthesized by reverse transcription using short fragments as templates, and then the cDNA ends were repaired, A-tailed, and connected with UMI tags and sequencing connectors
Finally, the library was obtained by PCR amplification
Assembly: https://www.cottongen.org/species/Gossypium_hirsutum/CRI-TM1
Supplementary files format and content: tab-delimited text files include readcount values for each Sample …

Submission date

Mar 21, 2023

Last update date

Mar 14, 2024

Contact name

YingQiang Long

E-mail(s)

lyq1061699314@outlook.com

Organization name

School of Agricultural Sciences, Zhengzhou University

Street address

Science Avenue