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| Status |
Public on Mar 14, 2024 |
| Title |
Xu142fl_0_rep1 |
| Sample type |
SRA |
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| Source name |
ovule
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| Organism |
Gossypium hirsutum |
| Characteristics |
tissue: ovule
dpa: 0
genotype: Xu142fl
treatment: Unhandled
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| Extracted molecule |
total RNA |
| Extraction protocol |
1 μg of total RNA was used to generate the sequencing libraries, which were sequenced using the Illumina platform by Beijing novogene Co., Ltd. (www.novogene.com, Beijing, China).
RNA libraries were prepared for sequencing using standard Illumina protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
The mRNA with polyA tail was enriched by Oligo (dT) magnetic beads.
Fragmentation buffer was added to randomly break the mRNA into short fragments.
The cDNA was synthesized by reverse transcription using short fragments as templates, and then the cDNA ends were repaired, A-tailed, and connected with UMI tags and sequencing connectors
Finally, the library was obtained by PCR amplification
Assembly: https://www.cottongen.org/species/Gossypium_hirsutum/CRI-TM1
Supplementary files format and content: tab-delimited text files include readcount values for each Sample …
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| Submission date |
Mar 21, 2023 |
| Last update date |
Mar 14, 2024 |
| Contact name |
YingQiang Long |
| E-mail(s) |
lyq1061699314@outlook.com
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| Organization name |
School of Agricultural Sciences, Zhengzhou University
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| Street address |
Science Avenue
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| City |
Science Avenue |
| ZIP/Postal code |
450001 |
| Country |
China |
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| Platform ID |
GPL29713 |
| Series (1) |
| GSE227870 |
Histone H3 lysine 27 trimethylation (H3K27me3) modulates the fiber initiation in upland cotton |
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| Relations |
| BioSample |
SAMN33845668 |
| SRA |
SRX19742271 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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