|
| Status |
Public on Feb 16, 2012 |
| Title |
normal mouse fallopian tube oviduct, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
normal mouse oviduct
|
| Organism |
Mus musculus |
| Characteristics |
disease state: normal
tissue: oviduct
|
| Growth protocol |
We used a mouse genetics strategy to conditionally delete both Dicer and the Pten tumor suppressor in the female reproductive tract using anti-Müllerian hormone receptor type 2 (Amhr2-Cre). These Dicer-Pten DKO (Dicer flox/flox Pten flox/flox Amhr2 cre/+) mice universally develop high-grade serous epithelial cancers.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the mirVana miRNA Isolation Kit (ABI). RNA quality were inspected on a 2100 Bioanalyzer (Agilent).
|
| Label |
biotin
|
| Label protocol |
According to Illumina protocol.
|
| |
|
| Hybridization protocol |
According to Illumina protocol.
|
| Scan protocol |
According to Illumina protocol.
|
| Description |
normal mouse fallopian tube oviduct, biological rep1
|
| Data processing |
The image data were compiled into a project using Beadstudio software gene expression module version 3.2.7. No normalization was used. The data was exported as txt files using the build-in function of export to GeneSpring GX format. The option of sample probe profile was chosen. Data were subsequently quantile normalized for the analysis.
|
| |
|
| Submission date |
Apr 19, 2011 |
| Last update date |
Feb 16, 2012 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE28720 |
Gene expression analysis of ovarian tumors from mice with knockout of DICER and PTEN |
| GSE28979 |
Gene expression profiles of serous ovarian cancer in human and mouse |
|
