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Sample GSM7123319 Query DataSets for GSM7123319
Status Public on Apr 03, 2023
Title Sham1-cerebrospinal fluid
Sample type protein
 
Source name Cerebrospinal fluid of rat
Organism Rattus norvegicus
Characteristics strain: Cerebrospinal fluid
gender: Male
treatment: Untreated
Extracted molecule protein
Extraction protocol none provided
Label Cy3
Label protocol Cy3-conjugated streptavidin
 
Hybridization protocol Centrifuge the Cy3-streptavidin tubule, then add 1.4 ml of sample diluent, mix well, and then quickly centrifuge again. Add 80 ยต l of Cy3-streptavidin to each well, wrap the slide in aluminum foil and incubate it in a dark place. Incubate it on a shaking table at room temperature for 1 hour.
Scan protocol The signals (532 nm excitation, 635 nm emission) were scanned and extracted using an InnoScan 300 scanner (Innopsys, Carbonne, France).
Description Secreted proteins
Data processing Individual array spots were background subtracted locally by subtracting the median background across spot replicated in each sample, and then normalized using two positive controls.
QAR-INF-1 software was used to analyze the data
 
Submission date Mar 29, 2023
Last update date Apr 04, 2023
Contact name Han Li
E-mail(s) lihanxinyi@126.com
Organization name Xinxiang Medical University
Street address 601 Jinsui Road, Hongqi District, Xinxiang City, Henan Province
City Xinxiang
ZIP/Postal code 453003
Country China
 
Platform ID GPL33293
Series (1)
GSE228521 10 Inflammatory Factors in Cerebrospinal Fluid and Serum of Parkinson's Disease Rats

Supplementary file Size Download File type/resource
GSM7123319_Sham1-CSF.txt.gz 267 b (ftp)(http) TXT
Processed data are available on Series record

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