|
Status |
Public on Apr 03, 2023 |
Title |
PD+human menstrual blood-derived endometrial stem cells4-CSF |
Sample type |
protein |
|
|
Source name |
Cerebrospinal fluid of rat
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Cerebrospinal fluid gender: Male treatment: PD+MenSCs-Treated
|
Extracted molecule |
protein |
Extraction protocol |
none provided
|
Label |
Cy3
|
Label protocol |
Cy3-conjugated streptavidin
|
|
|
Hybridization protocol |
Centrifuge the Cy3-streptavidin tubule, then add 1.4 ml of sample diluent, mix well, and then quickly centrifuge again. Add 80 ยต l of Cy3-streptavidin to each well, wrap the slide in aluminum foil and incubate it in a dark place. Incubate it on a shaking table at room temperature for 1 hour.
|
Scan protocol |
The signals (532 nm excitation, 635 nm emission) were scanned and extracted using an InnoScan 300 scanner (Innopsys, Carbonne, France).
|
Description |
Secreted proteins
|
Data processing |
Individual array spots were background subtracted locally by subtracting the median background across spot replicated in each sample, and then normalized using two positive controls. QAR-INF-1 software was used to analyze the data
|
|
|
Submission date |
Mar 29, 2023 |
Last update date |
Apr 04, 2023 |
Contact name |
Han Li |
E-mail(s) |
lihanxinyi@126.com
|
Organization name |
Xinxiang Medical University
|
Street address |
601 Jinsui Road, Hongqi District, Xinxiang City, Henan Province
|
City |
Xinxiang |
ZIP/Postal code |
453003 |
Country |
China |
|
|
Platform ID |
GPL33293 |
Series (1) |
GSE228521 |
10 Inflammatory Factors in Cerebrospinal Fluid and Serum of Parkinson's Disease Rats |
|