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Sample GSM7134253 Query DataSets for GSM7134253
Status Public on Oct 01, 2023
Title MPF-R1 RET
Sample type RNA
 
Source name MPFR cells
Organism Homo sapiens
Characteristics cell line background: MCF-7 cells
cell type: MPFR cells
genotype: RET siRNA
Treatment protocol MPFR and TPFR were established through long-term treatment with 100 nM fulvestrant and 200nM palbociclib over a period of 2-4 months.
Growth protocol MPFR cells were cultured in phenol red-free Dulbecco’s modified Eagle medium (D-MEM)/F-12 (Gibco) supplemented with 1% heat-inactivated fetal bovine serum (FBS, Gibco), 2 mM glutamine (Gibco) and 6 ng/ml insulin (Sigma-Aldrich) supplemented with 200 nM CDK4/6i and 100 nM fulvestrant. TPFR cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 media without phenol red (Gibco, ThermoFisher Scientific, Waltham, MA, USA), supplemented with 5% FBS, 2 mmol/L glutamine, 8 µg /mlL insulin and 200 nM CDK4/6i and 100 nM fulvestrant.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified with RNeasy Mini kit (Qiagen) according to the manufacturer´s instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human transcriptome array 2.0 (HTA). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from MPFR RET-siRNA
CAlv-Ext-MPF-R1-ret-HTA-2_0-a1.sst-rma-combined-dabg.chp Signal
Data processing Data analysis was conducted in Transcriptome Analysis Console (Thermo Fisher Scientific).
 
Submission date Mar 31, 2023
Last update date Oct 01, 2023
Contact name Carlos Maria Lourenco Alves
E-mail(s) calves@health.sdu.dk
Phone +4591101707
Organization name SDU
Street address J.B. Winsløws vej 25 3.
City Odense
ZIP/Postal code 5000
Country Denmark
 
Platform ID GPL17586
Series (1)
GSE228637 Microarray gene expression data from RET siRNA-mediated knockdown in CDK4/6 inhibitor breast cancer resistant cells

Data table header descriptions
ID_REF
VALUE sst-rma-combined-dabg.chp Signal

Data table
ID_REF VALUE
TC0X002348.hg.1 5.46
TC0X002349.hg.1 5.4
TC0X002358.hg.1 6.08
TC0X002353.hg.1 5.92
TC03001345.hg.1 5.47
TC17001753.hg.1 12.61
TC0X002354.hg.1 5.91
TC0X002351.hg.1 5.82
TC10001579.hg.1 4.8
TC04001824.hg.1 12.73
TC20000913.hg.1 12.8
TC21000289.hg.1 13.25
TC0X002226.hg.1 4.47
TC0X002355.hg.1 5.48
TC04001139.hg.1 11.9
TC21000004.hg.1 8.19
TC07001751.hg.1 10.85
TC0X000738.hg.1 4.43
TC0X002315.hg.1 5.4
TC0X000568.hg.1 4.74

Total number of rows: 67528

Table truncated, full table size 1394 Kbytes.




Supplementary file Size Download File type/resource
GSM7134253_CAlv-Ext-MPF-R1-ret-HTA-2_0-a1.CEL.gz 26.7 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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