|
Status |
Public on Apr 30, 2023 |
Title |
Synchronized L4/young adult hlh-30 animals grown on 2% glucose - 4 |
Sample type |
SRA |
|
|
Source name |
Whole extract
|
Organism |
Caenorhabditis elegans |
Characteristics |
tissue: Whole extract developmental stage: L4/young adult genotype: hlh-30(tm1978) treatment: 2% glucose
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Trizol-chloroform followed by purification with Quiagen RNA purification kit. Libraries were prepared using NEBNext Ultra II RNA Library Prep by Illumina. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
hlh_gluc_4
|
Data processing |
Raw data of fastq format were processed through in-house perl scripts. The clean data were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data. All the downstream analyses were based on the clean data with high quality. gene_count.xls generated using featureCounts 1.5.0-p3 Assembly: WBcel235 Supplementary files format and content: gene_count.xls of raw gene counts
|
|
|
Submission date |
Apr 03, 2023 |
Last update date |
Apr 30, 2023 |
Contact name |
Marcas Agostinelli |
E-mail(s) |
marcus.agostinelli@gmail.com
|
Phone |
8144047650
|
Organization name |
Novogene
|
Department |
Bioinformatics
|
Street address |
2690 W Avenida Azahar
|
City |
Tucson |
State/province |
Arizona |
ZIP/Postal code |
85745 |
Country |
USA |
|
|
Platform ID |
GPL26672 |
Series (1) |
GSE228784 |
RNA seq to determine differential gene expression in pgph-2 oe C.elegans animals and the subset of HLH-30-dependent genes on normal growth and excess glucose conditions. |
|
Relations |
BioSample |
SAMN34046437 |
SRA |
SRX19847796 |